The colonies are usually raised and have a waxy appearance, which develop radial furrows and irregular folds. The most commonly isolated trichosporon species was trichosporon asahii 57 of 76, 75%. Trichosporon cutaneum is a dimorphic fungus initially isolated from a dairy factory and. Trichosporon cutaneum has been previously known as oidium cutaneum. Metabolism of aromatics by trichosporon oleaginosus while. The yeast strains cryptococcus podzolicus, trichosporon porosum and pichia segobiensis were isolated from soil samples and identified as oleaginous yeast strains beneficial for the establishment of microbial production processes for sustainable lipid production suitable for several industrial applications. An trichosporon cutaneum in nahilalakip ha genus nga trichosporon, ngan familia nga trichosporonaceae. Wo 20151648 a1 agents and methods of treatment the. This article is from brazilian journal of microbiology, volume 41. Small amounts of yeast extract and nh 4 cl were included in the medium to achieve an initial carbon. Influence of various phenolic compounds on phenol hydroxylase. Dimorphism of trichosporon cutaneum and impact on its lipid.
Six human pathogenic trichosporon species are described with respect to criteria for routine identification, epidemiology and clinical origin. In particular, the name trichosporon beigelii is now obsolete, and previously described infections reported in the literature under this name could in fact be due to any one of the species listed below. The phenoldegrading strain trichosporon cutaneum r57 utilizes various aromatic and aliphatic compounds as a sole carbon and energy source. Epidemiology and diagnosis of mycotic infections in canine otitis externa. We performed by the end of 2015 a systematic search in the pubmed database using the following key words. The authors report the case of a leukemic patient who developed splenic infection from disseminated t. Oct 17, 2016 intraperitoneal fluconazole therapy for trichosporon cutaneum peritonitis in continuous ambulatory peritoneal dialysis. When cultured in bioreactors with glucose as the sole carbon source c. Yeast trichosporon cutaneum on deproteinized whey in. Despite the reported increase of trichosporon infections refractory to. Trichosporon asahii, a noncandida yeast that caused fatal septic shock in a patient without cancer or neutropenia.
Nov 17, 2017 the oleaginous yeast, trichosporon oleaginosus, has been extensively studied for its ability to metabolize nonconventional feedstocks. At the time of transplantation, intraoperative cultures of pericardial and mediastinal fluid. She had a stormy course despite adequate antimicrobial therapy. Biodegradation of phenol at high concentration by a novel. Invasive infection due to trichosporon cutaneum is a significant complication in hematologic malignancies. Trichosporon is a genus of anamorphic fungi in the family trichosporonaceae.
The intracellular activities of phenol hydroxylase ec 1. Infection with trichosporon pullulans annals of internal. All species of trichosporon are yeasts with no known teleomorphs sexual states. The oleaginous yeast, trichosporon oleaginosus, has been extensively studied for its ability to metabolize nonconventional feedstocks. Characterization of newly isolated oleaginous yeasts. Genome profiling gp as an effective tool for monitoring. Odds f c, arai t, disalvo a f, evans e g v, hay r j, randhawa h s, rinaldi m g, walsh t j. We describe the first human infection with a related organism, t. In this study, we found that the oleaginous yeast strain trichosporon cutaneum as 2. Different toxic phenol derivatives cresols, nitrophenols and.
Invasive trichosporonosis it is an emerging fatal opportunistic infection and it predominantly occurs in immunocompromised hosts. Molecular identification of trichosporon species pdf paperity. Trichosporon trikosporon, trikosporon, a genus of imperfect fungi that possess branching septate hyphae with arthroconidia and blastoconidia. Trichosporon species have emerged as important human pathogens during the past 4 decades in association with an increased number of immunocompromised hosts. The basidiomycetous yeast trichosporon may cause severe. Fungemia caused by trichosporon beigelii cutaneum has been recently recognized as a fatal infection afflicting immunocompromised patients. Text is available under the creative commons attributionsharealike license. Pdf dimorphism of trichosporon cutaneum and impact on its. Trichosporon cutaneum is a yeastlike fungus that causes white piedra and onychomycosis.
Although most species are considered commensals of the human skin and gastrointestinal tract, these basidiomycetes are an increasing cause of fungal disease among immunocompromised hosts, such as hematological patients and solid organ transplant recipients. This novel species was considered a sibling species of the cutaneum clade. A 62yearold woman with chronic active hepatitis receiving immunosuppressive drugs was initially admitted with sepsis secondary to an intraabdominal abscess caused by klebsiella pneumoniae. Trichosporon mycotoxinvorans is used to detoxify mycotoxins. An initial blast search suggests this yeast has putative aromatic metabolizing genes.
Review spoilage yeasts in the wine industry ulisboa. These species are causative agents of white piedra and cutaneous infections and are involved in systemic, localized or disseminated mycoses. Appressoria lateral swellings on hyphae that function that function in attachment are variably present. Trichosporon cutaneum isolated from a case of otomycosis. Simultaneous utilization of glucose and xylose for lipid production by trichosporon cutaneum by cuimin hu, siguo wu, qian wang, guojie jin, hongwei shen and zongbao k zhao download pdf 389 kb. Trichosporon mycotoxinivorans, a novel respiratory pathogen in patients with cystic fibrosis. Trichosporon cutaneum trichosporon pullulans microscopic appearance hyphae septate, hyaline. Trichosporon is a genus of anamorphic yeasts basidiomyceta, hymenomycetes, trichosporonales with a distinct morphological characteristic of true mycelia that disarticulate to form arthroconidia. Rectangular arthroconidia are normally prominent, with pseudohyphae and blastoconidia present in fewer numbers, but the relative ratios vary. Trichosporon spp an emerging fungal pathogen medicina. Presentation mode open print download current view. Recently, it has also been recognized as an opportunistic pathogen in immunocompromised hosts. Simultaneous utilization of glucose and xylose for lipid.
The etiology of disseminated t, beigelii infection. Trichosporon cutaneum definition of trichosporon cutaneum. Product monograph ketoderm cream 2% ketoconazole, usp topical antifungal agent. Trichosporon, trichosporonosis, invasive infection, sepsis, deepseated infection and fungemia. The diagnosis of postoperative fungal endophthalmitis, with rare exceptions, has been made on the. Trichosporon beigelii or trichosporon cutaneum finding. Trichosporon pullulans trichosporon cutaneum hanseniapora uvarum trycosporum pullulans candida zeylanoides a species presented by decreasing level of occurrence. Proliferation of trichosporon yeasts in the hair can lead to an unpleasant but nonserious condition. Dimorphism of trichosporon cutaneum and impact on its. Trichosporon species are ureasepositive, nonencapsulated basidiomycetous yeasts characterised by the development of hyaline, septate hyphae that fragment into oval or rectangular arthroconidia. This report shows that the plasma glucan concentration was also elevated in four patients infected with fusarium, trichosporon beigelii, saccharomyces cerevisiae and acremonium. Principles and practice of clinical mycology, chichester.
A 44yearold man with endstage ischemic cardiomyopathy was supported with an intraaortic balloon and the penn state heart artificial prior to orthotopic cardiac transplantation on the 14th hospital day. Comprehensive stool test advanced laboratory testing. Trichosporon asahii an overview sciencedirect topics. They are found in soil and water and are known to colonize skin and gastrointestinal tract of humans 1, 2. Disseminated trichosporon beigelii cutaneum infection in an. Trichosporon cutaneum can metabolize pentose sugars more efficiently, and in the meantime is. After the extensive revision of the taxonomy of trichosporon spp.
Successful treatment of disseminated trichosporon beigelii. Most are typically isolated from soil, but several species occur as a natural part of the skin microbiota of humans and other animals. Figure 1a shows the impact of yeast extract supplementation on glucose consumption, cell growth and lipid accumulation of t. Deep systemic trichosporon infection has been observed in association with hematologic malignancy, central venous catheter use, broadspectrum antibiotics, neutropenia, receiving of antineoplastic chemotherapy, immunosuppressive therapy and diminished immune function of various causes 11,15. Trichosporon beigelii is a species of fungus in the family trichosporonaceae. Download endnote the crystal structure of phenol hydroxylase in complex with fad and phenol provides evidence for a concerted conformational change in the enzyme and its cofactor during catalysis. Invasive infections with trichosporon beigelii also called cutaneum or t. Trichosporon species isolated from the perigenital region. Trichosporon cutaneum is a dimorphic fungus initially isolated from a dairy factory and, not.
Aug 24, 2011 table 1 results of lipid production by trichosporon cutaneum cultivated on glucose and xylose mixtures full size table moreover, as shown in table 1, sugarconsumption rates were roughly proportional to their individual concentrations in the medium, which led to complete consumption of both sugars at about the same time figure 2ac. Dimorphism of trichosporon cutaneum and impact on its lipid production. Alternatively, you can download the file locally and open with any standalone pdf reader. They colonize the skin and gastrointestinal tract of humans. A method for inhibiting growth or infestation of a pathogen in or on a human or animal subject, said method comprising contacting the pathogen with an effective amount of a combination of a plant defensin or a functional natural or synthetic derivative or variant thereof and either. However, this species experiences dimorphic transition between yeastform cells and hyphae during submerged fermentation, which consequently affects. The probe binds to various regions of genome dna with mismatch andor bulgecontaining structures as operated under lower stringency conditions, leading to the generation of a set of dna fragments. This organism is usually resistant to antifungal agents. Molecular identification of trichosporon species pdf. Data from antifungal susceptibility testing ast studies were also. Trichosporon cutaneum, and exceptionally trichosporon asahii and trichosporon loubieri 2, 6. Pdf characterization of trichosporon species isolated. An trichosporon cutaneum in uska species han fungi in nahilalakip ha divisio nga basidiomycota, ngan nga syahan ginhulagway ni beurm.
The microbial strain is precultured on 1 liter of the medium by shaking at 30c for 2 days. Cell extracts were prepared from trichosporon cutaneum grown with phenol or p cresol, and activities were assayed for enzymes catalyzing conversion of these. Trichosporon species trichosporon capitatum trichosporon cutaneum trichosporon pullulans microscopic appearance hyphae septate, hyaline. Onychomycosis caused by trichosporon cacaoliposimilis. The phenol hydroxylase from trichosporon cutaneum hydroxylates phenol to catechol. To potentially detect speciesspecific epidemiological traits, we limited our search after 1994, date of the revised. An initial blast search suggests this yeast has putative aromatic metabolizing. Abiotrophia species or granulicatella species finding 710322003, snomedct acinetobacter alcaligenes or acinetobacter lwoffi finding 717196000, snomedct acinetobacter anitratus or acinetobacter haemolyticus finding 717194002, snomedct acinetobacter baumannii or acinetobacter calcoaceticus or acinetobacter pittii finding. Disseminated trichosporon beigelii cutaneum infection in. Invasive trichosporon cutaneum infection in an infant with. Abstractthe present study aimed to 1 determine the colonization rates of medically important.
Pdf phenol utilization by filamentous yeast trichosporon cutaneum. The genus, in general, consists of soilassociated species, although eight species are causative agents of diseases in man 1,2,7. Yeast growth and lipid synthesis are regulated by nutritional conditions, particularly nitrogen limitation, through which amino acid metabolism is regulated to redirect carbon flux to lipid synthesis. These include phenolcontaining waste streams, such as distillery wastewater, or streams consisting of nonconventional sugars, such as hydrolyzed biomass and various bagasse. Trichosporon beigelii is associated with white piedra. Malfeitoferreira international journal of food microbiology 86 2003 2350. It has been reported that some strains of bacteria, or fungi,, especially certain strains of yeasts,, are capable of converting the phenol to the carbon dioxide and the water and are tolerant to the toxicity of the phenol at concentration of 500 mgl or even higher. Cell extracts were prepared from trichosporon cutaneum grown with phenol or pcresol, and activities were assayed for enzymes catalyzing conversion of these. Phenol is the best substrate, but the enzyme also accepts simple hydroxyl, amino, halogen or methylsubstituted phenols. Pdf the investigated strain trichosporon cutaneum shows well expressed capability for metabolizing high concentrations of phenol, up to 1. Mar 18, 2014 the yeast strains cryptococcus podzolicus, trichosporon porosum and pichia segobiensis were isolated from soil samples and identified as oleaginous yeast strains beneficial for the establishment of microbial production processes for sustainable lipid production suitable for several industrial applications.
This pdf is available for free download from a site hosted by. Disseminated trichosporon beigelii cutaneum infection in an artificial heart. Aug 29, 2019 compared to the oleaginous yeast yarrowia lipolytica, trichosporon cutaneum can metabolize pentose sugars more efficiently, and in the meantime is more tolerant to inhibitors, which is suitable for lipid production from lignocellulosic biomass. Recent research, however, has shown that several trichosporon species are involved in. Frontiers the cell biology of the trichosporonhost. Download fulltext pdf characterization of trichosporon species isolated from clinical specimens in kuwait article pdf available in journal of medical microbiology 54pt 7. This led to the use of two names for this fungus with clinical relevance. It is a yeast that was formerly considered to be the cause of an unpleasant hair condition called white piedra and also the cause of a more serious severe opportunistic infection trichosporonosis in immunocompromised individuals. Pdf dimorphism of trichosporon cutaneum and impact on. Feb 12, 2018 trichosporon species are fungi that commonly inhabit the soil. Trichosporon cutaneum an overview sciencedirect topics. Trichosporon at the us national library of medicine medical subject headings mesh template. How do i view different file formats pdf, doc, ppt, mpeg on this site. Trichosporon behrend is a genus of anamorphic yeasts yeast can be isolated from the sputum, the urine, the skin and.
113 1142 1523 338 1301 985 796 591 876 1202 981 1234 912 1204 1329 20 1544 708 999 11 1038 270 1224 1073 334 743 695 1082 1474 45 1463 278 334